alicator ligation independent cloning (lic) and expression kit (Thermo Fisher)
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alicator ligation independent cloning (lic) and expression kit
Alicator Ligation Independent Cloning (Lic) And Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alicator+ligation+independent+cloning+and+expression+system/alicator+ligation+independent+cloning+and+expression+system/pm40181138-38-7-9
Average 90 stars, based on 1 article reviews
Alicator Ligation Independent Cloning (Lic) And Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alicator+ligation+independent+cloning+and+expression+system/alicator+ligation+independent+cloning+and+expression+system/pm40181138-38-7-9
Average 90 stars, based on 1 article reviews
alicator ligation independent cloning (lic) and expression kit - by Bioz Stars,
2026-10
90/100 stars
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Polymerase Chain Reaction:Article Title: Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment. Article Snippet: .. Obtained PCR products were cloned into pLate52 and pLate31 vectors, respectively for the NH-his-tagged and CH-his-tagged variants, using an Article Title: Enzymatic Synthesis of a Polyketide/Nonribosomal Peptide Hybrid Antibiotic, Salivabactin. Article Snippet: Salivabactin is a newly identified polyketide/nonribosomal peptide (PK/NRP) from a human oral probiotic, possessing a unique chemical structure and outstanding antibiotic activities.. Although the biosynthetic gene cluster for salivabactin is known, the enzymatic logic that governs the synthesis of salivabactin has not yet been fully studied.. In this work, we dissected the biosynthetic pathway for salivabactin using biochemical analysis. Clone Assay:Article Title: Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment. Article Snippet: .. Obtained PCR products were cloned into pLate52 and pLate31 vectors, respectively for the NH-his-tagged and CH-his-tagged variants, using an Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Article Title: Genome Sequencing and Assembly of Enterotoxigenic Escherichia coli E9034A: Role of LngA, CstH, and FliC in Intestinal Cell Colonization and the Release of the Proinflammatory Cytokine IL-8 Article Snippet: .. The lngA , cstH , and fliC genes were cloned and inserted into the pLATE31 plasmid according to the Ligation:Article Title: Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment. Article Snippet: .. Obtained PCR products were cloned into pLate52 and pLate31 vectors, respectively for the NH-his-tagged and CH-his-tagged variants, using an Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Article Title: Enzymatic Synthesis of a Polyketide/Nonribosomal Peptide Hybrid Antibiotic, Salivabactin. Article Snippet: Salivabactin is a newly identified polyketide/nonribosomal peptide (PK/NRP) from a human oral probiotic, possessing a unique chemical structure and outstanding antibiotic activities.. Although the biosynthetic gene cluster for salivabactin is known, the enzymatic logic that governs the synthesis of salivabactin has not yet been fully studied.. In this work, we dissected the biosynthetic pathway for salivabactin using biochemical analysis. Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Article Title: The incC Sequence Is Required for R27 Plasmid Stability Article Snippet: .. The recombinant plasmid for C-terminal His-tagged R27-encoded E protein overexpression was obtained following the instructions of Article Title: Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins Article Snippet: .. After amplification with mlrBF and mlrBR primers , mlrB was inserted into pLATE31 vector using an Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Article Title: Genome Sequencing and Assembly of Enterotoxigenic Escherichia coli E9034A: Role of LngA, CstH, and FliC in Intestinal Cell Colonization and the Release of the Proinflammatory Cytokine IL-8 Article Snippet: .. The lngA , cstH , and fliC genes were cloned and inserted into the pLATE31 plasmid according to the Cloning:Article Title: Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment. Article Snippet: .. Obtained PCR products were cloned into pLate52 and pLate31 vectors, respectively for the NH-his-tagged and CH-his-tagged variants, using an Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Article Title: Enzymatic Synthesis of a Polyketide/Nonribosomal Peptide Hybrid Antibiotic, Salivabactin. Article Snippet: Salivabactin is a newly identified polyketide/nonribosomal peptide (PK/NRP) from a human oral probiotic, possessing a unique chemical structure and outstanding antibiotic activities.. Although the biosynthetic gene cluster for salivabactin is known, the enzymatic logic that governs the synthesis of salivabactin has not yet been fully studied.. In this work, we dissected the biosynthetic pathway for salivabactin using biochemical analysis. Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Article Title: The incC Sequence Is Required for R27 Plasmid Stability Article Snippet: .. The recombinant plasmid for C-terminal His-tagged R27-encoded E protein overexpression was obtained following the instructions of Article Title: Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins Article Snippet: .. After amplification with mlrBF and mlrBR primers , mlrB was inserted into pLATE31 vector using an Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Article Title: Genome Sequencing and Assembly of Enterotoxigenic Escherichia coli E9034A: Role of LngA, CstH, and FliC in Intestinal Cell Colonization and the Release of the Proinflammatory Cytokine IL-8 Article Snippet: .. The lngA , cstH , and fliC genes were cloned and inserted into the pLATE31 plasmid according to the Binding Assay:Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Amplification:Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Article Title: Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins Article Snippet: .. After amplification with mlrBF and mlrBR primers , mlrB was inserted into pLATE31 vector using an Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Derivative Assay:Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Isolation:Article Title: Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody production. Article Snippet: .. The SARS-CoV-2 receptor binding domain (RBD) was amplified from a template of cDNA derived from a clinical isolation of SARS-CoV-2 and cloned into pLATE-31 according to the protocol of the Plasmid Preparation:Article Title: Enzymatic Synthesis of a Polyketide/Nonribosomal Peptide Hybrid Antibiotic, Salivabactin. Article Snippet: Salivabactin is a newly identified polyketide/nonribosomal peptide (PK/NRP) from a human oral probiotic, possessing a unique chemical structure and outstanding antibiotic activities.. Although the biosynthetic gene cluster for salivabactin is known, the enzymatic logic that governs the synthesis of salivabactin has not yet been fully studied.. In this work, we dissected the biosynthetic pathway for salivabactin using biochemical analysis. Article Title: The incC Sequence Is Required for R27 Plasmid Stability Article Snippet: .. The recombinant plasmid for C-terminal His-tagged R27-encoded E protein overexpression was obtained following the instructions of Article Title: Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins Article Snippet: .. After amplification with mlrBF and mlrBR primers , mlrB was inserted into pLATE31 vector using an Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Article Title: Genome Sequencing and Assembly of Enterotoxigenic Escherichia coli E9034A: Role of LngA, CstH, and FliC in Intestinal Cell Colonization and the Release of the Proinflammatory Cytokine IL-8 Article Snippet: .. The lngA , cstH , and fliC genes were cloned and inserted into the pLATE31 plasmid according to the Transformation Assay:Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Purification:Article Title: Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 2019 Article Snippet: .. The SARS-CoV-2 receptor-binding domain (RBD) was amplified from the cDNA of a SARS-CoV-2 clinical isolate and cloned in pLATE-31 according to the protocol of the Recombinant:Article Title: The incC Sequence Is Required for R27 Plasmid Stability Article Snippet: .. The recombinant plasmid for C-terminal His-tagged R27-encoded E protein overexpression was obtained following the instructions of Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the Over Expression:Article Title: The incC Sequence Is Required for R27 Plasmid Stability Article Snippet: .. The recombinant plasmid for C-terminal His-tagged R27-encoded E protein overexpression was obtained following the instructions of Expressing:Article Title: Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A Article Snippet: Restriction enzymes were purchased from Promega (Promega Corporation, WI, USA) and were used according to the manufacturer's recommendations. .. Primer design, amplification, cloning and expression of lngB and lng genes in the pLATE31 plasmid were conducted according to the |